Journal: Journal of Cellular Biochemistry

Article Title: Targeting PKC-Mediated Signal Transduction Pathways Using Enzastaurin to Promote Apoptosis in Acute Myeloid Leukemia-Derived Cell Lines and Blast Cells

doi: 10.1002/jcb.23090

Figure Lengend Snippet: PKC β and PKC δ expression is generally elevated IN AML blast cells compared to normal CD34+ bone marrow cells. Real-time PCR was performed as described in Materials and Methods section. Expression of PRKCA (PKC α), PRKCB (PKC β), PRKCD (PKC δ), and PRKCE (PKC ε) genes are presented as relative to 100 copies of b2M for AML-derived cell lines (A) and as relative to 100 copies of ABL1for CD34+ cells derived from bone marrow from normal donors (N = 6) and blast cells derived from AML patients (N = 75; B). P values were derived as described in Materials and Methods section. Statistically significant differences from expression in normal bone marrow cells (P < 0.05) are marked by “*.”

Article Snippet: Statistical analysis was performed with Sigma Stat computer software (SSPS, Chicago, IL). table ft1 table-wrap mode="anchored" t5 TABLE II caption a7 Common name Symbol Chromosome location ABI assay ID PKC α PRKCA 17q22-q23.2 Hs00176973_m1 PKC β PPP2R1B 16p11.2 Hs00176998_m1 PKC δ PPP2R2A 3p21.31 Hs00178914_m1 PKC ε PPP2R5A 2p21 Hs00178455_m1 β-2-microglobulin B2M 15q21-q22.2 Hs99999907_m1 ABL1 ABL1 9q34.1 Hs00245445_m1 Open in a separate window List of PKC Genes and ABL1 and B2M Control Analyzed Using Real-Time PCR With Applied Biosystems (ABI) Taqman Assays

Techniques: Expressing, Real-time Polymerase Chain Reaction, Derivative Assay

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet: Neutralizing mAbs as pre-exposure prophylaxis in k18-hACE2 mice CV1-1, CV1-30, and CV2-75 were assessed to see whether they could confer protection in a mouse model. (A) Experimental timeline. (B) Number of PFUs in the lungs 2 days following challenge. Error bars indicate SD. (C) Viral RNA in lung tissue 2 days after challenge was measured by qPCR and normalized to GAPDH expression. Error bars indicate SD. (D) Kaplan-Meyer survival curve of the viral load/titer in the lungs of remaining mice comparing the various treatment groups. Statistics were determined by one-way ANOVA with Dunnett’s multiple comparison test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Expressing, Comparison

Journal: Cell Reports

Article Title: Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects

doi: 10.1016/j.celrep.2021.109353

Figure Lengend Snippet:

Article Snippet: The following Taqman Primer/Probe sets (Thermo Fisher Scientific) were used in this study: Gapdh (Mm99999915_g1).

Techniques: Virus, Infection, Recombinant, Affinity Column, Purification, Staining, Cloning, Cell Isolation, Transfection, Luciferase, Reverse Transcription, Gene Expression, Nested PCR, Sequencing, Expressing, Plasmid Preparation, Software, Microscopy